Kem chromatography yog ib txoj kev siv dav rau kev sib cais thiab ua kom huv si ntawm cov tshuaj sib xyaw. Kev ntsuas ntawm kem chromatography suav nrog ntau yam tseem ceeb uas ua kom muaj kev sib cais zoo. Ntawm no yog ib qho piv txwv ntawm kev ntsuas:
1. Kem
Khoom siv:Feem ntau yog ua los ntawm iav, stainless hlau, los yog yas.
Loj:Nws txawv raws li qhov ntsuas ntawm chromatography (kev tshuaj ntsuam, kev npaj, lossis kev lag luam).
Packed nrog:Nyob ruaj khov theem (piv txwv li, silica gel, alumina, los yog cob).
Frit los yog Lim:Tiv thaiv cov theem nyob ruaj ruaj los ntawm kev tawm hauv kem nrog rau theem mobile.
2. Theem nyob ruaj ruaj
Kev sib xyaw:Khoom siv nrog tshwj xeeb adsorptive zog.
Hom:Silica gel, alumina, los yog cov khoom siv ua haujlwm (xws li, ion-exchange lossis affinity cov ntaub ntawv).
3. Phase Txawb
Solvent lossis Solvent Mixture:Nqa tus qauv mus rau theem nyob ruaj khov.
Hom:Tsis-polar (piv txwv li, hexane), polar (xws li, methanol, dej), los yog gradient ntawm polarity.
4. Sample Injector
Siv los qhia cov qauv sib xyaw rau hauv kab.
Yuav ua tau ib phau ntawv txhaj tshuaj los yog ib tug automated system rau meej tswj.
5. Pump (yeem)
Xyuas kom zoo ib yam ntawm cov theem mobile.
Siv nyob rau hauv high-performance kua chromatography (HPLC) los yog thaum muaj meej tswj ntawm ntws yog xav tau.
6. Ntes (Yeem)
Txheeb xyuas thiab txheeb xyuas cov khoom sib cais hauv lub sijhawm tiag tiag thaum lawv hais tawm ntawm kab.
Piv txwv muaj xws li:
UV-Vis Spectrophotometer
Fluorescence Detector
Mass Spectrometer (MS)
Refractive Index (RI) Detector
7. Fraction Collector
Sau cov feem sib cais ntawm cov tebchaw raws li lawv elute los ntawm kem.
Tej zaum yuav suav nrog kev sau tsis siv neeg raws lub sijhawm lossis lub teeb liab ntes.
8. Data Acquisition System (Yeem)
Sau thiab txheeb xyuas cov ntaub ntawv los ntawm tus ntes.
Tsim cov chromatograms uas tso saib cov txheej txheem sib cais.
9. Cov khoom siv
Siab Regulators:Tiv thaiv over-pressurization.
Thermostats los yog Heaters:Tswj qhov kub thiab txias, yog tias tsim nyog.
Valves:Tswj qhov ntws ntawm cov theem mobile thiab cov qauv.
Rau kev teeb tsa yooj yim (piv txwv li, lub ntiajteb txawj nqus-raws li kab chromatography), cov twj tso kua mis, cov cuab yeej ntes, thiab cov tshuab tsis siv neeg yuav tsis tsim nyog. Hloov chaw, lub ntiajteb txawj nqus los yog lub tshuab tsis tshua muaj siab tsav lub sijhawm txawb ntawm lub sijhawm nyob ruaj khov.