Txoj kev rau Refining Cov Txiav Txim Siab

Txoj kev rau Refining Cov Txiav Txim Siab

Chromatographic sib cais yog cov txheej txheem uas nquag siv rau kev muab cov chrome rho tawm, muab cov chromatography, ion txauv cov chromatography, thiab gel chromatography.

⑴adsorption chromatography siv adsorbents zoo li silica gel lossis alumina los cais cov khoom sib txawv raws cov sib txawv raws sib txawv.

⑵partition chromatograph cais cov tshuaj raws li lawv cov kev faib cov coefficients hauv ob {}}} pase kuab tshuab.

⑶ion txauv cov chromatography yog qhov tsim nyog rau kev them cov tshuaj them rau kev ua kom huv.

⑷gel chromatography cais cov molecules los ntawm qhov loj me.

Cov hau kev no tuaj yeem siv tau ntawm ib tus zuj zus lossis ua ke, nyob ntawm lub hom phiaj sib xyaw ua ke cov khoom, muab kev sib cais ntau thiab xaiv tau.

Crystallization yog ib txoj kev classic rau refining cov lus sib txuas, ua tiav purification los ntawm kev sib cais los ntawm kev sib cais los ntawm cov txheej txheem crystallization. Cov kauj ruam tseem ceeb suav nrog:

⑴ELSELECTION TSAV TXIV TXWV,

⑵heating thiab dissolving qhov sib txuas,

⑶performing kub pom kom tshem tawm impurities,

⑷controlling crystallization tej yam kev mob.

Recrystallization Txuas ntxiv ua kom huv huv cov khoom crude crystalline, txhim kho kev coj dawb huv. Hom no yog tsim tshwj xeeb rau cov tebchaw nrog cov chaw kub - Vam khom solubility thiab yog qhov tseem ceeb nyob rau hauv cov tshuaj ntxiv.

⑴liquid -} Ua kua extraction cais cais cov tshuaj raws li cov sib txawv ntawm ob qho kev sib faib ntawm cov kuab tshuaj tiv thaiv.

⑵supercrritical kua rho tawm (piv txwv li, siv cov khoom siv hluav taws xob ua haujlwm) yog cov thev naus laus zis ib puag ncig, thiab siv thev naus laus zis ib puag ncig.

⑶aqueous ob -} theem ntu extraction yog qhov zoo tagnrho rau cais biomacromolecules.

Cov hau kev no yooj yim ua haujlwm thiab feem ntau yog ua kev lim dej ua ntej kom tshem tawm cov impurities.

Adsorption kev vam khom rau kev xaiv adsorbents xws li ua haujlwm carbon lossis macroporous resins:

⑴Tevated carbon feem ntau yog siv rau decolorization thiab deodorization.

⑵MACROPOLOE resins resins adsorb cov hom phiaj raws li polarity thiab molecular loj.

Txoj hauv kev no yog cov cuab yeej siv - Tus phooj ywg thiab dav siv rau hauv cov khoom ntuj extraction, tshwj xeeb yog rau kev ua kom muaj kev ua ntej.

Membrane sib cais muaj cov micbrictrictration, ultrefiltration, nanofiltration, thiab rov qab osmosis, uas cais cov lwg me me los ntawm qhov loj me. Cov ntsiab lus muaj xws li:

⑴Mnild kev khiav hauj lwm (tsis muaj theem / tshuaj hloov pauv),

⑵stability rau cua sov -} Cov tshuaj uas rhiab,

Iffective tshem tawm cov me me - molecule tsis kam thiab phiaj siab.

Nws yog dav siv nyob rau hauv biologics thiab kws tshuaj paus.

Lim cov khoom xav tau cov hau kev tsim nyog:

⑴vacuum kom qhuav (kev siv dav dav),

⑵Creeze Kom qhuav (khaws cia cua sov - rhiab rexounds),

⑶spray ziab (loj - nplai ntau lawm).

Cov kauj ruam ntxiv xws li milling thiab sieving ua kom cov khoom homogeneity.

Nruj kev tswj hwm muaj kev tswj hwm tau cuam tshuam ntau xws li:

⑴Chromatography (HPLC, GC, TLC),

⑵melting kev txiav txim siab,

⑶smentroscopic tsom xam.

Kev ua kom dawb huv, cov ntsiab lus tsis huv, thiab cov kev seem thiab cov chaw seem yuav tsum tau raws cov qauv kev cai. Ib lub zog tswj tau zoo yog qhov tseem ceeb rau kev ua tau zoo.

Qhov kev xaiv ntawm cov qauv ua kom zoo yog nyob ntawm:

⑴target cov khoom lag luam,

⑵⑵Prity Profile,

⑶production nplai.

{- Cov nplai sim ua kom zoo ua cov txheej txheem ua ntej ntsuas. Kev phaj sib npaug, tawm los, nqi, kev nyab xeeb, thiab ib puag ncig cuam tshuam yog qhov tseem ceeb.